Real-Time Single Cell Analysis
Engineering of single-cell-level cell fate investigation and modulation
Trends in the dynamic response of a cell leads a number of new approaches in understanding a cell. Specifically, the combination of a microfluidic device with time-lapse imaging techniques allows us to monitor the cellular response at a single-cell level. Moreover, since the micro-environment surrounding the cells in vivo is dynamic rather than static, dynamic stimulation is crucial.
Taking advantage of various techniques such as live cell imaging, mathematical modeling and micro fabrication, we suggest studying heterogeneity of a cell fate by measuring signaling events at single cell level. Ultimately, our approach aims to take advantage of these systems properties to engineer robustness in the control of cell fate. In this regard, we developed an integrated platform providing spatial-temporal dynamic stimulation in various profiles. Also, our group recently succeeded in-frequency modulation of cell fate decision using the microfluidic device, giving us a novel conceptual approach to regenerative medicine and stem cell therapy.
Our vision is to develop a new approach, a new analysis, and a new insight that have never been tried with the conventional techniques. Beyond providing novel biological insight in stem cell biology, we also provide an initial technological framework to use our approach to manipulate cell fate in large amounts of cells.
Peer-Reviewed Journal Publications
"Frequency modulation of ERK activation dynamics rewires cell fate"
Hyunryul Ryu, Minhwan Chung, Maciej Dobrzynski, Dirk Fey, Yannick Blum, Sung Sik Lee, Matthias Peter, Boris N Kholodenko, Noo Li Jeon and Olivier Pertz
Molecular Systems Biology, 11(11), 838-855 (2015)